Journal: PloS one

Article Title: Dysferlin interacts with tubulin and microtubules in mouse skeletal muscle.

doi: 10.1371/journal.pone.0010122

Figure Lengend Snippet: Figure 6. Dysferlin binds to microtubules. His-myc-dysferlin purified on Ni-NTA beads was incubated with polymerized microtubules. Reactions were resolved by SDS-PAGE stained with SimplyBlue SafeStain. Arrows point to His-myc-dysferlin, tubulin, BSA, and to MAP1&MAP2 of the microtubule-associated protein fraction (MAPF), which includes MAP2A, MAP2B, MAP1 and tau. S: Soluble phase, P: Pellet. Lane 1: Microtubules alone, lane 2: Microtubules incubated with MAPF, lane 3: Microtubules incubated with BSA, lane 4: MAPF alone, lane 5: BSA alone, lane 6: Purified His-myc- dysferlin alone, lane 7: Microtubules incubated with purified His-myc-dysferlin. doi:10.1371/journal.pone.0010122.g006

Article Snippet: BSA was used as a negative control and the microtubule binding fraction (MAPF) provided by the cytoskeleton assay kit containing MAP2A, MAP2B, MAP1 and tau as a positive control, where MAP2 constitutes 70% of MAPF.

Techniques: Purification, Incubation, SDS Page, Staining

Journal: Neuron

Article Title: A dopamine gradient controls access to distributed working memory in the large-scale monkey cortex

doi: 10.1016/j.neuron.2021.08.024

Figure Lengend Snippet: (A) i: 109 cortical regions of the Julich Macaque Brain Atlas, identified by receptor and cytoarchitecture. ii:D1 receptor density. The receptor density shown here does not take into account differences in neuron density across areas. (B) i: divided the macaque cortex into 42 slabs of tissue, here mapped on to the Yerkes19 surface. ii: neuron density across the cortex. (C) i: injection sites for the studies of dendritic spine density by . ii: number of dendritic spines on the basal dendrites of layer III pyramidal cells. (D) i: 40 injected areas in the retrograde tract-tracing database of . ii: cortical hierarchy. (E) Circular embedding of the cortical hierarchical connectivity structure. Radial distance to the center represents the hierarchical position of the area, with the areas lowest in the hierarchy closest to the edge. Angular distance between areas represents the inverse of connectivity strength (fraction of labeled neurons - FLN), so that areas that are plotted at similar angles are more strongly connected to each other. Colors represent the angle on the circle. Clear visual and somatosensory hierarchies emerge from this circular embedding of the connectivity data (highlighted with arrows). Association areas lie at angles off the main visual and somatosensory hierarchies. (F) The density of D1 receptors divided by neuron density. Regions that have not yet been measured are shown in gray. (G) There was a strong positive correlation between the D1 receptor density per neuron and the cortical hierarchy. The spatially corrected p value is the fraction of randomly generated surrogate maps with spatial smoothness matched to the hierarchy map that show a stronger Pearson correlation (negative or positive) with the D1 receptor map than the hierarchy map itself. See also and .

Article Snippet: For each area in the Lyon atlas, we searched for overlaps with areas in the Julich Macaque Brain Atlas.

Techniques: Injection, Labeling, Generated

Journal: Neuroimage

Article Title: Organization of the macaque monkey inferior parietal lobule based on multimodal receptor architectonics

doi: 10.1016/j.neuroimage.2021.117843

Figure Lengend Snippet: Location and extent of IPL areas in the macaque monkey brain. A : 3D reconstruction of the left hemisphere (in lateral view) of Macaca mulatta (DP1) brain obtained using Connectome Workbench software ( https://www.humanconnectome.org/software/connectome-workbench ) showing the location and extent of the areas that compose the IPL (map made publicly available at https://balsa.wustl.edu/study/7qgpZ). The enlarged reconstruction scheme indicates the approximate positions of cut plane (a-i) for a series of exemplary coronal sections, which were processed for the visualization of topography of IPL areas. B - E : Low magnification views of representative fields of IPL areas taken from the four exemplary coronal sections. For each section, the dashed boxes indicate the location of the photomicrographs; arrows indicate the borders between cytoarchitectonic areas. The cytoarchitectonic subdivisions of the IPL convexity, areas Opt, PG, PFG and PF, are shown in B, C, D and E , respectively. The opercular area PGop is shown in C , PFop are shown in D and E . Dorsal is up and lateral on the right. Scale bar, 500 μm.

Article Snippet: Upon publication, the ensuing 3D map of the macaque IPL, and the receptor fingerprints will be made publicly available to the neuroscientific community via the Human Brain Project and BALSA repositories.

Techniques: Software

Journal: Neuroimage

Article Title: Organization of the macaque monkey inferior parietal lobule based on multimodal receptor architectonics

doi: 10.1016/j.neuroimage.2021.117843

Figure Lengend Snippet: Comparison of laminar receptor densities of kainate receptor in IPL areas. A : Coronal sections through four caudo-rostral levels of a macaque hemisphere showing kainate receptor distribution patterns in the IPL. The borders between the IPL areas (red lines) are charted on the pseudocolor-coded autoradiographs. The color bar beneath each autoradiograph indicates receptor concentrations by the different colors, from dark blue for low to red for high concentrations (fmol/mg protein). B : Laminar distribution pattern of representative fields of IPL areas. Roman numerals indicate cytoarchitectonic layers. C : Bar plot shows receptor densities in each layer for the IPL areas, separately. Receptor densities (y axis) are given in fmol/mg protein. Brackets across bars denote the areas which have significant inter-area differences in laminar densities (* p < 0.05, ** p < 0.01).

Article Snippet: Upon publication, the ensuing 3D map of the macaque IPL, and the receptor fingerprints will be made publicly available to the neuroscientific community via the Human Brain Project and BALSA repositories.

Techniques: Comparison, Autoradiography

Journal: Neuroimage

Article Title: Organization of the macaque monkey inferior parietal lobule based on multimodal receptor architectonics

doi: 10.1016/j.neuroimage.2021.117843

Figure Lengend Snippet: The absolute mean areal densities and laminar densities of seven exemplary receptor types (i.e. GABA B , GABA A /BZ, M 2 , α 1 , 5-HT 1A , 5-HT 2 and D 1 ) have been projected onto the corresponding IPL areas. Color bars code for receptor densities in fmol/mg protein. The projections of other receptor types onto the Yerkes 19 surface are shown in S12 Figure. The file coding for the densities of all 15 receptors in each area is provided as Supplementary data 1, and is made available to the neuroscientific community via the Human Brain Project (https://kg.ebrains.eu/search/instances/Project/e39a0407-a98a-480e-9c63-4a2225ddfbe4) and BALSA (https://balsa.wustl.edu/study/7qgpZ) repositories.

Article Snippet: Upon publication, the ensuing 3D map of the macaque IPL, and the receptor fingerprints will be made publicly available to the neuroscientific community via the Human Brain Project and BALSA repositories.

Techniques:

Journal: Neuroimage

Article Title: Organization of the macaque monkey inferior parietal lobule based on multimodal receptor architectonics

doi: 10.1016/j.neuroimage.2021.117843

Figure Lengend Snippet: A : Receptor fingerprints of the examined IPL areas. Absolute densities (fmol/mg protein) of 15 receptors are displayed in polar coordinate plots (scaling 0-2500 fmol/mg protein) of 6 brain areas. The corresponding transparent surface indicates the standard deviation. The positions of the different receptor types and the axis scaling are identical in all polar plots, and specified in the polar plot at the bottom right corner of the figure. B : Receptor-driven clustering of the monkey IPL areas. Hierarchical cluster analysis reveals 2 receptor-architectonically distinct clusters: a caudal cluster (red) consisting of areas Opt, PG and PGop; and a rostral cluster (blue) consisting of areas PFG, PF and PFop. C : Multi-Dimensional Scaling (MDS) analysis of the monkey IPL areas. The distances between areas represent the Eigenvalues of the first and second dimensions, two clusters are segregated by the first dimension, same color coding as in A .

Article Snippet: Upon publication, the ensuing 3D map of the macaque IPL, and the receptor fingerprints will be made publicly available to the neuroscientific community via the Human Brain Project and BALSA repositories.

Techniques: Standard Deviation

Journal: Neuroimage

Article Title: Organization of the macaque monkey inferior parietal lobule based on multimodal receptor architectonics

doi: 10.1016/j.neuroimage.2021.117843

Figure Lengend Snippet: Comparative analysis of human and macaque IPL areas. A : Multidimensional scaling analysis reveals the segregation of human and macaque areas by the first dimension, whereas the second dimension reflects changes in receptor fingerprints along the rostro-caudal axis of each species. Note, that to facilitate interpretation of the results, the MDS plot has been rotated counter-clockwise by 90° and flipped horizontally. Thus, human areas are found at the top of the plot and macaque areas at the bottom. Rostral areas are to the left, caudal ones to the right. Dashed lines with arrows connect homolog areas. B : Schematic representation of the topographic relationship between IPL areas in the human (top) and macaque (bottom) brains. Colour coding represents clusters as revealed by hierarchical cluster analyses, the detailed information of macaque clusters can be found in Figure 6 . Three receptor-architectonically distinct clusters in human were found by Caspers S et al. : a caudal cluster (red) consisting of areas PGp and PGa; an intermediate cluster (green) consisting of areas PFm and PF, and a rostral cluster (blue) consisting of areas PFt, PFcm and PFop. Abbreviations: ips intraparietal sulcus, lf lateral fissure.

Article Snippet: Upon publication, the ensuing 3D map of the macaque IPL, and the receptor fingerprints will be made publicly available to the neuroscientific community via the Human Brain Project and BALSA repositories.

Techniques: